Anti-Apolipoprotein B (human)
Mouse monoclonal antibody
Cat.No. HYB 069-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent:0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.
Human LDL adsorbed onto aluminum hydroxide gel
HYB 069-02 is specific for apo B from human serum/plasma. No cross-reactivity is seen with human apolipoprotein A-1.
Epitope differs from that of HYB 069-03 and HYB 069-04
HYB 069-02 reacts with apo B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against apo B, and also with isolated LDL directly coated onto the microtiter plate.
In Western blotting after SDS-PAGE HYB 069-02 binds to apo B in reduced as well as in non-reduced forms, using isolated LDL as antigen.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Micic S, Arends J, Norgaard-Pedersen B, Christoffersen K, Andersen GE (1988) Simultanous quantification by double rocket immunoelectrophoresis of apoliproteins A-1 and B in blood spotted on filter paper. Clin Chem 34:2452-2455.
2. Young SG, Smith RS, Hogle DM, Curtiss LK, Witztum JL (1986) Two new monoclonal antibody-based enzyme-linked assays of apolipoprotein B. Clin Chem 32:1484-1490.
