Anti-Immunoglobulin light chain (rat, kappa chain)

Mouse monoclonal antibody

Cat.No. MARK 1

Subclass IgG1/k
Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Secreted immunoglobulins (Ig) have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen.

Immunogen:

Immunoglobulin kappa light-chain from rat

Specificity:

MARK 1 is specific for the pmmunoglobulin kappa light-chain in rats.

Cross-reactivity has not been determined

Epitope specificity:

Not determined

Reactivity:

MARK-1 reacts with the immunoglobulin kappa light-chain from rat in ELISA.

Application:
Method Usability References
ELISA Yes
Immunoblotting Not determined
Immunohistochemistry Not determined
References:

1. Bazin H, Xhurdebise LM, Burtonboy G, Lebacq AM, Clercq LD, Cormont F (1984) Rat Monoclonal Antibodies. I. Rapid Purification from In Vitro Culture Supernatants. J Immunol Methods 66:261-269.
2. Brogren CH, Hirsch F, Wood P, Druet P, Poussier P (1986) Production and characterization of a monoclonal islet cell surface autoantibody from the BB rat. Diabetologia 29:330-333.

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