Anti-Influenza B virus nucleoprotein
Mouse monoclonal antibody
Cat.No. HYB 116-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza B virus is a basic, phosphorylated multimeric protein consisting of 560 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle (1).
Unpurified influenza B virus (B/Lee/40) for primary intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37°C (2).
HYB 116-02 reacts with influenza B virus nucleoprotein as demonstrated by immunoblotting after SDS-PAGE. HYB 116-02 does not cross-react with influenza A virus (2).
Epitope differs from HYB 116-03 as determined by inhibition ELISA
HYB 116-02 is suitable as capture antibody in combination with biotinylated HYB 116-03 as detecting antibody in sandwich ELISA for influenza B virus (2).
HYB 116-02 reacts with influenza B-infected, acetone-fixed VERO cells in immunofluorescence cytochemistry.
In Western blotting a dilution guideline of 1/20 has proved successful (2).
| Method | Usability | References |
| ELISA | Yes | 2 |
| Immunoblotting | Yes | 2 |
| Immunoflourescence | Yes |
1. Portela A, Digard P (2002) The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J Gen Virol 83:723-734
2. Glikmann G, Chen S-N, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization. Clin Diagn Virol 4:27-42.
Larger Picture (124kB)
HYB 116-02 diluted 1/1000 with a secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1:20 + 1% Evans blue colors Influenza B nucleoprotein. Primarily the nucleus of the infected Vero cells is visible
Related Articles
Antibody MSDS
April 23 2008
