Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Corynebacterium diphtheriae is a Gram-positive, non-motile bacterium that can cause serious illness in unvaccinated humans. C. diphtheriae infects the epithelial cells of the upper respiratory tract from where they secrete a potent toxin that is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body. The toxin is a single protein chain of 62 kDa which is "nicked" at proteolytic processing sites to produce an A chain of 21 kDa and a B chain of 40 kDa, held together by a disulfide bridge. The A-chain carries the ADP-ribosylation enzymatic activity responsible for the toxic action (1).

Diphtheria toxoid prepared from toxin purified from C. diphtheriae subsp. intermedius Park-Williams no. 8 by formaldehyde inactivation (2), and adsorbed to aluminum hydroxide gel.

HYB 123-05 reacts with diphtheria toxoid (3) and also neutralizes diphtheria toxin as determined in a VERO cell assay.

Not determined

HYB 123-05 reacts strongly with diphtheria toxoid. HYB 123-05 detects diphtheria toxoid in sandwich ELISA in combination with a polyclonal antibody against the toxoid as capture antibody (3). Strong reaction is also seen in ELISA with diphtheria toxoid coated directly onto the microtiter well.

An antibody pair consisting of HYB 123-09 and HYB 123-05 can be used in a sandwich ELISA when using HYB 123-09 as a coat and biotinylated HYB 123-05 a detection antibody.