Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Leptin, also called obesity factor, is a protein produced by adipose tissue, which has a regulatory influence on food intake, energy metabolism and reproduction. Leptin may also play a role in immune and inflammatory responses and hematopoiesis

Recombinant human leptin adsorbed onto aluminium hydroxide gel

ABS 022-11 binds human leptin

Cross-reactivity with other species has not been determined

The epitope of ABS 022-11 differs from the epitope of ABS 022-03 but overlaps with the epitope of ABS 022-06

ABS 022-11 binds free leptin in solution. The ELISA dilution guideline applies to leptin coated directly at 1.0 mg/mL. In sandwich ELISA, bioyinylated ABS 022-11 works as a detection antibody with ABS 022-03 as capture antibody.

In Western blotting 3 bands are visible at 16, 32 and 48 kDa corresponding with the leptin monomer, dimer and trimer forms.