BioPorto Diagnostic

AntibodyShop

Anti-Gastric Inhibitory Polypeptide (GIP)

Mouse monoclonal antibody

Cat.No. ABS 021-04

Subclass IgG1/k

Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Human gastric inhibitory polypeptide (GIP) is a 42 amino acid peptide belonging to the glucagon-secretin family of peptide hormones. It is secreted by endocrine cells in the duodenal mucosa and stimulates glucose-dependent insulin secretion as well as GLP-1 release from more distal endocrine (L) cells in the intestinal mucosa.
GIP shows amino-acid sequence similarities to glucagon, GLP-1 and GLP-2 (from approximately 50% identity for glucagon to 30% identity for GLP-2).

Immunogen:

Synthetic human gastric inhibitory polypeptide adsorbed onto aluminum hydroxide gel

Specificity:

ABS 021-04 binds human GIP. ABS 021-04 cross-reacts up to 10% with PACAP and human GLP-2 but <0.1% with glucagon, GLP-1 or VIP.

Cross-reactivity with porcine GIP has not been determined.

Epitope specificity:

Not determined

Reactivity:

The dilution guideline applies to glutaraldehyde-linked GIP-OA (molar ratio 4:1) coated at 1 µg/mL. With GIP coated directly at 2 µg/mL the dilution guideline is 1/2000. In addition ABS 021-04 binds free GIP in solution.

Application: