Anti-alpha-calcitonin gene-related peptide (alpha-CGRP)
Mouse Monoclonal Antibody
Cat.No. ABS 026-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Calcitonin gene-related peptide is a 37 amino acid vasoactive neuropeptide (MW 3789) widely expressed throughout the central and peripheral nervous system. CGRP has potent vasodilatory effects probably serving as a regulator of regional blood flow, in addition to a variety of other cellular effects.
Synthetic human alpha-CGRP adsorbed onto aluminum hydroxide gel
ABS 026-05 binds human alpha-CGRP. Based on inhibition ELISA, ABS 026-05 shows full cross-reactivity with rat alpha-CGRP and about about 50% cross-reactivity with human beta-CGRP. Cross-reactivity with coated rat alpha-CGRP is low (8%) and there is no cross-reaction with human amylin.
The epitope lies within residues 1-18 of human alpha-CGRP
ABS 026-05 binds free alpha-CGRP in solution. Based on inhibition ELISA, the affinity of ABS 026-05 is at least 24 times higher than that of ABS 026-04.
The ELISA dilution guideline applies to human alpha-CGRP coated directly at 1.0g/mL. showing that the antibody binds poorly to the denatured peptide.
ABS 026-05 (as capture antibody) forms a sandwich ELISA pair with ABS 026-04 (as biotinylated detection antibody) for measuring human alpha-CGRP, giving a detection limit of 0.13 nmol/L in an unoptimized buffer assay.
ABS 026-05 works well in IHC on paraffin embedded tissue.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Not determined | |
| Immunohistochemistry | Yes |
Larger Picture (365kB)
Paraffin embedded rat tissue stained with ABS 026-05 Anti- alpha CGRP diluted 1/400 in 1% BSA/PBS and incubated over night.
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Antibody MSDS
April 23 2008
