Anti-Complement factor H (human, ß1H-globulin)

Mouse monoclonal antibody

Cat.No. HYB 268-01

Subclass IgG1/k
Presentation:

Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Factor H is a regulatory factor of the alternative complement pathway. Factor H is a glycoprotein consisting of a single polypeptide chain with molecular mass of 155 kDa. Human plasma concentration is app. 500 µg/ml (1,2).

Immunogen:

Factor H isolated from human plasma adsorbed onto aluminum hydroxide gel

Specificity:

HYB 268-01 is specific for human factor H (ß1H-globulin)

Epitope specificity:

Not determined

Reactivity:

HYB 268-01 reacts strongly with factor H. A strong reaction is seen in sandwich ELISA in combination with a polyclonal antibody against factor H (eg. Abcam AB8842), when testing normal human serum. In Western blotting HYB 268-01 reacts with factor H.

Application:
Method Usability References
ELISA Yes
Immunoblotting Yes
Immunohistochemistry Not determined
References:

1. Law SKA, Reid KBM (1988) Complement In: In Focus (Ed. Male D) IRL Press: Oxford.
2. Bendtzen K et al (2000) Basal og klinisk immunologi. FADL´s Forlag A/S, Copenhagen.

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