Anti-Complement factor D (human), biotinylated
Mouse monoclonal antibody
Cat.No. GAU 008-01B
Preparation: Biotinylated
Content: 100 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.
Human factor D (1).
GAU 008-01 is specific for complement factor D in human serum and plasma (1).
Epitope specificity differs from that of GAU 010-04 (1).
GAU 008-01 is used as a biotinylated detection antibody in sandwich ELISA with a GAU 010-04 capture (1). GAU 008-01 can be used for Western blotting following SDS-PAGE under non-reducing conditions (2).
| Method | Usability | References |
| ELISA | Yes | 1 |
| Immunoblotting | Yes | 1, 2 |
| Immunohistochemistry |
1. Oppermann M, Baumgarten H, Brandt E, Gottsleben W, Kurts C, Gotze O (1990) Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods 133:181-190.
2. Oppermann M, Kurts C, Zierz R, Quentin E, Weber MH, Gotze O (1991) Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure. Kidney Int. 40:939-947.
3. Oppermann M, Höpken U, Götze O (1992) Assessment of complement activition in vivo. Imm. pharm 24:119-134.
Downloads
GAU 008-01B.pdf (29.96 kB) Product specification
ELISA Kits and Monoclonal antibodies for the Complement system - flyer.pdf (637.76 kB)
ELISA Kits and Monoclonal antibodies for the Complement system
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