Anti-H-Ficolin (human)
Mouse monoclonal antibody
Cat.No. RIG 334-01
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human
serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by
carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by
means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa
protein chains associated in triplet subunits which are again associated in high molecular weight
oligomers by means of disulfide bonds at the N-terminal region.
Recombinant human H-ficolin
RIG 334-01 reacts specifically with H-ficolin. No cross-reaction has been observed with L- or M-ficolin
(1).
Not determined
RIG 334-01 reacts with recombinant H-ficolin coated directly onto the microtiter well. A sandwich ELISA
for H-ficolin uses RIG 334-01 for both capture and detection (1).
| Method | Usability | References |
| ELISA | Yes | 1 |
| Immunoblotting | Not determined | |
| Immunohistochemistry | Not determined |
1. Munthe-Fog L, Hummelshøj T, Ma YJ, Hansen BE, Koch C, Madsen HO, Skjødt K, Garred P (2008)
Characterization of a polymorphism in the coding sequence of FCN3 resulting in a Ficolin-3 (Hakata antigen)
deficiency state. Molecular Immunology 45:2660-2666.
Downloads
RIG 334-01.pdf (29.83 kB) Product Specification
Elisa kits and Monoclonal antibodies for the Complement system flyer.pdf (478.39 kB)
ELISA Kits and Monoclonal antibodies for the Complement system
