Anti-Antithrombin III (human, native)
Mouse monoclonal antibody
Cat.No. HYB 230-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.
Antithrombin III isolated from human plasma adsorbed onto aluminum hydroxide gel (1)
HYB 230-04 is specific for human AT III, native form
Epitope specificity differs from that of HYB 230-01, HYB 228-10 and HYB 228-12.
HYB 230-04 reacts strongly with native AT III. A strong reaction is seen in ELISA with native AT III coated directly onto the microtiter well, and also when used as detection antibody in sandwich ELISA in combination with a polyclonal capture antibody (eg. DAKO A 296). HYB 230-04 reacts with AT III in Western blotting after SDS-PAGE.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Sorensen PJ, Ezban M, Jessen TE, Sas G, Blasko G, Jorgensen M (1990) Purification and characterisation of the pathological antithrombin III "Aalborg". Thromb Res 57:807-812.
